2021 Fiscal Year Final Research Report
Functional analysis of Runx3 in fibroblasts within breast cancer
Project/Area Number |
19K16754
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 50010:Tumor biology-related
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Research Institution | Juntendo University |
Principal Investigator |
okubo shoki 順天堂大学, 医学部, 助手 (20827878)
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Project Period (FY) |
2019-04-01 – 2022-03-31
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Keywords | RUNX3 / 乳癌 / 癌微小環境 / CAFs |
Outline of Final Research Achievements |
CAFs, which are abundant in the cancer microenvironment, act on nearby breast cancer cells to promote malignant transformation of cancer, but their molecular mechanism is not fully understood. In this study, RUNX3-shRNA was introduced into human breast cancer-derived CAFs in order to analyze the role of Runx3 in the cancer-promoting ability of CAFs. It was suggested that the decreased expression of RUNX3 in CAFs significantly suppressed the proliferation of human breast cancer cells co-transplanted in immunodeficient mice. In the future, we plan to investigate whether the decrease in the cancer-promoting ability of CAFs due to the suppression of RUNX3 expression is due to the decrease in TGF-b-Smad2 / 3 and Wnt-b-catenin signals.
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Free Research Field |
消化器内科
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Academic Significance and Societal Importance of the Research Achievements |
CAFsがどの様に癌促進能を維持しているのかは不明な点が多い。本研究はCAFsでTGF-bオートクラインシグナルを媒介する遺伝子群を明らかにし、RUNX3がどのようにTGF-bやWnt-b-cateninシグナルの活性化に寄与するかを明らかにして、将来のCAFsを標的にした新規癌治療法の開発に役立てることを目的としている
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