Establishment of a method for the establishment of long-term lung cancer 3D organoid passaging lines and their clinical application

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K16764
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 50010:Tumor biology-related
• Research Institution: Tohoku University
• Principal Investigator: Notsuda Hirotsugu 東北大学, 大学病院, 助教 (00636037)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 肺がん3Dオルガノイド

Outline of Final Research Achievements

The purpose of this study is to develop and establish novel experimental methods that contribute to translational research (bridging research) and personalized medicine for cancer. Through the research period, we were able to establish a novel experimental method using lung cancer 3D organoids to some extent. It was also proved that the same experimental results as 2D models could be obtained using that experimental method, and we believe that this research was of great importance for future clinical applications. We hope to continue and develop this research because it can be applied to determine the efficacy of lung cancer treatment, except for immunotherapy, and to analyze the function of gene mutations.

Free Research Field

肺がん

Academic Significance and Societal Importance of the Research Achievements

本研究の成果によって、従来医学研究のために使用してきた2D細胞培養法とマウスを使用した動物実験に加えて第３の実験手法をある程度確立することができた。２D細胞培養法は、実験結果が真に生体で起こっている反応や環境を反映しているか評価が困難であるという欠点を有する。また動物実験には倫理的な問題と費用の問題が存在する。その点、研究者が開発に着手した3Dオルガノイドを用いた新規実験法は、それらの問題をクリアすることができる革新的な実験手法であり、その学術的意義や社会的意義は非常に大きいものと考える。