Investigation of the effectiveness of indocyanine green arterial injection for assessing intestinal blood flow in acute mesenteric ischemia

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K17198
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 52040:Radiological sciences-related
• Research Institution: Kobe University
• Principal Investigator: Sasaki Koji 神戸大学, 医学部附属病院, 助教 (10815989)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 急性腸間膜虚血症 / インドシアニングリーン / 動注

Outline of Final Research Achievements

We created two rabbit models of intestinal ischemia under analgesia and sedation. In rabbit 1, 25 mg of indocyanine green (ICG), a fluorescent dye, was administered by intravenous injection after dilution to five times its volume with 10 mL of injectable water. In rabbit 2, 25 mg of ICG was administered by arterial injection after dilution to 100 times its volume with 10 mL of injectable water. Ischemic intestines were observed using a fluorescent camera. Although intestinal blood flow was evaluable in both cases, due to the properties of ICG, sufficient dilution was necessary for imaging with the fluorescent camera, and intravenous administration with further dilution to reach the intestine in a more diluted state was considered desirable. Considering animal welfare, we deemed it unnecessary to continue further experiments and terminated the study after the two rabbits were tested.

Free Research Field

救急IVR

Academic Significance and Societal Importance of the Research Achievements

本研究では、急性腸管虚血症の血流評価においてインドシアニングリーン（ICG）の動脈注射の有効性を検証した。ICGを用いて腸管血流を評価することは、従来から研究されてきたが、本研究では、ICGを静脈内または動脈内に投与した場合の比較検討を行った。その結果、蛍光カメラによる腸管血流評価では、ICGをより希釈した状態で観察することが必要であり、従来から行われている静脈注射での評価で十分であることが分かった。