2020 Fiscal Year Final Research Report
Potential mechanism of Gadolinium penetration via intact blood-brain barriers and the effect of Gd retention to brain cancer proliferation
| Project/Area Number |
19K17224
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 52040:Radiological sciences-related
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| Research Institution | Gunma University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | Gadolinium / GBCA / Gadolinium retention / GDD / NSF / choroid plexus |
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| Outline of Final Research Achievements |
We found no significant differences in Gd concentration when Gadolinium-based contrast agents (GBCA) were incubated with transferrin. Incubation with Fe(II) did not cause any changes to the Purkinje cell arborization compared the control neurons. Fe(II) increased the effect of gadopentetate to the dendrite arborization, but it did not increase the effect of gadobutrol.
Fibrotic markers were increased in the skin on renal failure mouse model that was injected with gadodiamide, while only collagen 1α and TGF-β mRNA expression were higher in the gadopentetate group. NE activity in the blood serum and the expression of skin NE was significantly higher in the GBCA-treated mice compared to the control. We investigated the GBCA effect on the C6-astroglioma and TE761-medulloblastoma in-vitro. GBCAs significantly increase the C6 proliferation, but the effect was less significant in TE761 cells.
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| Free Research Field |
Gd造影剤, 放射線診断
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| Academic Significance and Societal Importance of the Research Achievements |
This project provides the information of:
The effect of Gd to the Purkinje cells, provide a background to reveal the mechanism of Gd retention in the brain, involvement of NE Skin lesion development in the Nephrogenic systemic fibrosis, and the effect of Gd to the proliferation of cancer cell lines.
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