2020 Fiscal Year Final Research Report
Development of the fetal therapy using maternal adipose-derived stem cells
Project/Area Number |
19K17378
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 52050:Embryonic medicine and pediatrics-related
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Research Institution | Showa University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | 胎児治療 / ダウン症 / 間葉系幹細胞 |
Outline of Final Research Achievements |
In utero stem cell transplantation is a promising medical tool for many genetic disorders, but graft rejection caused by maternal T cells as the main barrier to engraftment limits clinical outcomes. We hypothesized that using maternal adipose-derived stem cells (ADSCs) for in utero transplantation can develop maternal-fetal chimerism, thus lowering the risk of graft rejection. Herein, fetus brain engraftment using maternal grafts was examined via in utero stem cell transplantation. ADSCs were purified using MSC markers. Fetal brain grafts grew for at least 1 month after in utero transplantation and expressed mature neuronal markers. Furthermore, maternal ADSCs reduced immune cell infiltration and suppressed the innate immune response. In conclusion, in utero maternal ADSC transplantation is beneficial for the treatment of congenital CNS diseases because of the ability of the cells to differentiate into neuronal lineages and reduce immune responses.
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Free Research Field |
胎児治療
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Academic Significance and Societal Importance of the Research Achievements |
本研究では脂肪組織由来間葉系幹細胞が細胞外で神経細胞様の電位変化とタンパク質を発現することを確認し、さらに投与された胎児の脳組織内で神経細胞様に分化し生着することを明らかにしてきた。 胎児期に母体脂肪組織由来間葉系幹細胞 が生着した野生型マウスにおいて非投与群との間に明らかな運動性の異常などを認めないことを確認しているため、現在ダウン症モデルマウスでの機能改善を確認しく。胎児に対する幹細胞治療の臨床応用はその安全性と倫理的な問題から研究段階であるが、細胞リソースの特性や移植免疫応答を考えると母親から採取できる脂肪由来間葉系細胞を用いた胎児治療は応用が可能な幹細胞の一つとなると考ええ
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