Development of a novel angiogenic therapy using ultrasound-treated oxidative stress-resistant cells

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K17540
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Kurume University
• Principal Investigator: Takahiro Yoshikawa 久留米大学, 医学部, 助教 (70811082)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 血管新生療法 / 重症下肢虚血 / 皮下組織由来再生細胞

Outline of Final Research Achievements

Angiogenesis therapy, in which mesenchymal stem cells (ADRCs) residing in patients' subcutaneous adipose tissue are transplanted into ischemic tissue to promote collateral blood circulation, is attracting attention as a new treatment for critical limb ischemia. In this study, we attempted to enhance oxidative stress tolerance of angiogenic cells by applying ultrasound stimulation to the cells prior to administration. The results were compared under no sonication and sonication (Mechanical Index: MI 0.5 5 min/15 min, MI 1.2 5 min/15 min). 1.2 15 min MI resulted in a significant increase in HGF compared to the no sonication condition, although cell viability was reduced.

Free Research Field

血管新生療法

Academic Significance and Societal Importance of the Research Achievements

今回の研究結果は臨床用エコープローブによる超音波照射を用いて血管新生因子の産生を促せる可能性を示した初の報告であり、今後の血管新生療法の効果増強への寄与が期待される。