Omics approach for discovery of novel exosome biomarkers of heart failure

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K17583
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53020:Cardiology-related
• Research Institution: Fukuoka University (2020); National Cardiovascular Center Research Institute (2019)
• Principal Investigator: Nishigori Mitsuhiro 福岡大学, 理学部, 助教 (00633645)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: エクソソーム / 心不全 / バイオマーカー / ミトコンドリア / オミックス解析

Outline of Final Research Achievements

Since the number of patients with chronic heart failure is increasing in Japan, novel biomarkers useful for rapidly and accurate severity assessment and prognosis prediction are needed. In this study, we attempt to search for the biomarkers from proteins and RNAs which are included in exosomes released from cardiomyocytes. For this purpose, we established the protocols for exosome purification and sequential proteomic and transcriptomic analyses. In addition, we focused on the mitochondrial dynamics and disfunction in heart failure, and investigated the relationship between intracellular stress and mitochondrial abnormalities. As a result, it was suggested that the promotion of apoptosis by increasing oxidative stress might cause the myocardial damage.

Free Research Field

プロテオーム

Academic Significance and Societal Importance of the Research Achievements

本研究において、心筋から放出されるエクソソーム中に含まれるタンパク質が見出された。今後、これらの心不全における経時的な変動や心筋における機能の詳細が明らかとなれば、心不全の新規診断マーカーとして利用できる可能性が高い。また、本研究により確立したエクソソームの精製法ならびにオミックス解析方法は、心不全だけでなく他の疾患における新規バイオマーカー探索に広く活用できることが期待される。