Elucidating disease spectrum associated with pulmonary stem-cell dysfunction

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K17657
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53030:Respiratory medicine-related
• Research Institution: Department of Clinical Research, National Hospital Organization Kyoto Medical Center
• Principal Investigator: Yamamoto Yuki 独立行政法人国立病院機構(京都医療センター臨床研究センター), 展開医療研究部, 研究員 (10812133)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: 組織幹細胞 / 肺癌 / 肺線維症 / オルガノイド

Outline of Final Research Achievements

The purpose of this research was to elucidate the disease spectrum focusing on pulmonary stem-cell dysfunction, which is highly associated with intractable lung diseases. We established the workflow for recruiting lung samples from patients followed by histologic analysis focusing on tissue-stem cell associated markers as well as spatial omics analysis, which enables us to understand cellular heterogeneity and stem-cell specific niches. We also established methods for lung cancer organoids from patient samples, which can recapitulate the stem-cell niche abnormality in cancer tissue using spatial omics analysis.

Free Research Field

呼吸器内科学

Academic Significance and Societal Importance of the Research Achievements

本研究を通して、最近の肺幹細胞研究をリードする技術である先端的オミックス解析とiPS細胞モデルより得られた知見を、臨床検体をベースに融合させることが出来た。この研究により、肺胞幹細胞機能不全に基づく疾患スペクトラムの一端が解明され、将来的には共通ターゲットを標的とした疾患横断的治療への応用などが考えられ、今後急速にニーズが高まると想定されているプレシジョンメディシンへの貢献が期待できる。