Elucidation of factors that affect osteoblast differentiation of mesenchymal stem cells in renal failure

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K17753
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 53040:Nephrology-related
• Research Institution: Showa University
• Principal Investigator: Kato Tadashi 昭和大学, 医学部, 助教 (20644305)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: CKD-MBD / 間葉系幹細胞 / リン / 二次性副甲状腺機能亢進症

Outline of Final Research Achievements

Severe secondary hyperparathyroidism (SHPT) represents a high turnover bone disease, osteitis fibrosa, but the pathogenesis of osteitis fibrosa remains to be fully elucidated. We examined the characteristics of the differentiation of bone marrow mesenchymal stem cells (BMSCs) into osteoblasts in uremic rats. Osteoblastic differentiation markers in the BMSCs from the 5/6 nephrectomized rats were significantly suppressed compared with those isolated from the control groups. These results suggest that the BMSCs differentiation into osteoblasts was disturbed in the uremic rats.

Free Research Field

CKD-MBD

Academic Significance and Societal Importance of the Research Achievements

腎不全病態において間葉系幹細胞の骨芽細胞への分化能は通常の分化誘導環境においては低下しており、二次性副甲状腺機能亢進症における高回転性の骨代謝障害はカルシウムやリン、副甲状腺ホルモンといった生体内環境の要因が影響している可能性が示唆された。また腎不全病態における間葉系幹細胞の分化能へ変化を与える要因を今後調べることで、骨代謝障害の機序解明の一助となり得る。