New development of differentiation-inducing therapy using exosome derived from dedifferentiated adipocytes of neuroblastoma

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18072
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55010:General surgery and pediatric surgery-related
• Research Institution: Nihon University
• Principal Investigator: HOSHI Reina 日本大学, 医学部, 助手 (20793772)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 神経芽腫 / DFAT / Exosome

Outline of Final Research Achievements

By adding exosomes derived from dedifferentiated adipocytes (DFAT) to neuroblastoma cells and culturing them, the effect of suppressing the growth of tumor cells could not be obtained, but the differentiation of tumor cells could be induced. This is consistent with the previously shown differentiation-inducing effect of DFAT itself. Although DFAT has problems that must be solved before it can be used as cell therapy, using DFAT-derived exosomes for differentiation-inducing therapy has confirmed the possibility of using high-risk neuroblastoma with a poor prognosis in the future. We have found the possibility of leading to new treatments.

Free Research Field

小児外科

Academic Significance and Societal Importance of the Research Achievements

本研究では、ヒトDFATから放出されたエクソソームが神経芽腫の分化誘導効果を持つことが示された。神経芽腫は小児悪性固形腫瘍の中で脳腫瘍を除き、最も高頻度に認められ、高リスク群の神経芽腫は未だ予後不良である。今後、既存の神経芽腫に対する分化誘導療法である、13-cis RA（レチノイン酸）による分化誘導療法に、ヒトDFAT由来エクソソームを併用することで、分化誘導効果を向上させることが期待される。