Elucidation of cancer-related fibroblast appearance mechanism in colorectal cancer liver metastasis

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18103
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Keio University
• Principal Investigator: MONNO Masayoshi 慶應義塾大学, 医学部(信濃町), 助教 (60837681)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 癌幹細胞 / ニッチ

Outline of Final Research Achievements

It has been reported that fibrotic liver promotes liver metastasis of colorectal cancer, and this time, we focused on Lgr5-positive cells, which are cancer stem cells of colorectal cancer. Colorectal cancer cell lines with high Lgr5 expression promote liver metastasis in fibrotic liver more than cell lines with low Lgr5 expression, and Rspondin, a ligand for Lgr5, is more highly expressed in fibrotic liver. Was shown. When Rspondin was added to cell lines with high Lgr5 expression and cell lines with low expression of Lgr5 and cultured, cell proliferation was promoted in cell lines with high Lgr5 expression, and Rspondin in fibrotic liver had some effect on Lgr5-positive cells. It was considered that liver metastasis may be promoted by giving.

Free Research Field

消化器外科

Academic Significance and Societal Importance of the Research Achievements

Lgr5陽性細胞は大腸癌の癌幹細胞としての機能を有することが知られており、癌幹細胞として機能するための微小環境を形成するものの一つとしてRspondinが考えられました。大腸癌肝転移を抑制するための治療標的として期待できるとともに、肝転移メカニズムの解明の一助となる可能性が期待されます。