Ascites proteomics reveal the mechanism of pancreatic cancer peritoneal dissemination

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18107
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55020:Digestive surgery-related
• Research Institution: Tohoku University
• Principal Investigator: Takadate Tatsuyuki 東北大学, 大学病院, 助教 (50772216)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 膵癌 / プロテオミクス

Outline of Final Research Achievements

Stromal tissue of pancreatic cancer was selectively collected, and as a result of proteomics analysis, a protein called hemopexin involved in pancreatic cancer metastasis was identified(PLoS One. 2020 Jul). Examination of 146 staging laparoscopy of pancreatic cancer, metastasis was found in 24% of resectable pancreatic cancers. Body and tail cancer, cases with large tumor diameter, and cases with high CA19-9 values had significantly more metastases(Surg Today. 2021 May). Ascites of pancreatic cancer was collected and preserved in all cases of staging laparoscopy and laparotomy. Although it was not possible to comprehensively analyze the proteins expressed in ascites, CEA and CA19-9 in ascites were significantly higher in metastasis-positive cases, and tumor markers in ascites were higher than in serum. It was highly sensitive.

Free Research Field

膵癌

Academic Significance and Societal Importance of the Research Achievements

膵癌の間質組織のプロテオミクスから、分泌タンパク質であるヘモペキシンを同定し、報告したのは世界で初めてのことである。また審査腹腔鏡は進行膵癌では行われているが、切除可能膵癌も含めて全例で施行している施設は非常に稀であり、膵癌の様々な症例の貴重な腹水サンプルが採取・保存されている。
膵癌は治療方針決定のために、審査腹腔鏡を行うことが非常に重要と考えており、その際に腹水採取は容易であり、腹水を用いたリキッドバイオプシーは、非常に重要な検査となりうる。