Challenge to elucidate the pathogenesis of obesity paradox in pneumonia by microcirculatory physiology

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18251
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Department of Clinical Research, National Hospital Organization National Disaster Medical Center
• Principal Investigator: Mitsuda Shingo 独立行政法人国立病院機構災害医療センター(臨床研究部), 臨床研究部, 麻酔科医師 (80636431)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 肥満パラドックス / 肺炎 / 微小循環

Outline of Final Research Achievements

We created an obese mouse model, and weight differences were observed from 8 weeks of age in each group, and we were able to create a highly obese group, a moderately obese group, and a control group. We intubated mice and created a pneumonia model by spraying LPS through a tracheal tube, and observed a decrease in body weight and an increase in inflammatory response. We had planned to create a pneumonia model in obese mice and collect data in the final year of the project, but due to the spread of COVID-19 infection, it was difficult to conduct experiments due to restrictions on travel to experimental facilities and an increase in clinical work, and our research achievements were limited to the above.

Free Research Field

麻酔科学

Academic Significance and Societal Importance of the Research Achievements

肺炎は高齢者の死亡原因として常に上位に位置づけられ肺炎による死亡を減少させることは社会的なテーマとなっている。近年の研究によると、「BMI増加に伴 い死亡率が低下する」といういわゆる「肥満パラドックス」が肺炎病態でも見られるという分析結果がある。本研究で肥満マウスとコントロール(非肥満)マウス の肺炎病態下における微小循環レベルでの事象を「生体顕微鏡画像技術」を用いてin vivoで観察し、変化に富む生体肺血管内皮細胞の機能及び肺の組織学的変 化の差を明らかにし、肺炎における肥満パラドックスの病態生理学的機序を解明したいと考えた。