The study for improvement in diagnostic accuracy and elucidation of underlying mechanism of perioperative anaphylaxis by using basophil activation tests

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18286
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Gunma University
• Principal Investigator: Orihara Masaki 群馬大学, 医学部附属病院, 助教 (10795272)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 周術期管理学 / 周術期アナフィラキシー / 好塩基球活性化試験

Outline of Final Research Achievements

We investigated the optimal condition of blood samples during transportation for basophil activation tests. Further, we explored underlying mechanisms of perioperative drug-induced anaphylaxis. We found that the longer storage time and the higher storage temperature of the blood sample, the lower the basophil activation rate. In addition, an IgE-dependent pathway was suggested for perioperative anaphylaxis induced with some drugs.

Free Research Field

麻酔科学

Academic Significance and Societal Importance of the Research Achievements

好塩基球刺激試験(BAT)に用いる検体の取り扱い方法を最適化できれば、診断精度の向上が期待できる。さらに、アナフィラキシーの発症プロセスにおけるIgE関与の有無を調べることで、特異的IgE抗体測定による原因薬同定の可否が判明する。BATや特異的IgE抗体測定といった、in vitroの検査の診断精度を高めることで、患者を危険に曝すことなくアナフィラキシーの原因薬同定が可能となる。