Neuroprotective effects of sedative drugs in post-resuscitation encephalopathy using in vivo neuroactivity imaging

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18299
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55050:Anesthesiology-related
• Research Institution: Kagoshima University
• Principal Investigator: Nakahara Mayumi 鹿児島大学, 医歯学域医学系, 助教 (90707514)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 蘇生後脳症 / 炎症 / 神経活動イメージング

Outline of Final Research Achievements

When cardiopulmonary arrest results in hypoxia, the prognosis is severe due to organ damage that occurs after the return of spontaneous circulation. The purpose of this study was to investigate the effects of sedatives on post-resuscitation encephalopathy using cardiopulmonary arrest mice model. First, a fear conditioning test was performed using a post-operative pain model mouse to examine the evaluation method. We observed a freezing tendency and impaired memory retrieval in the anesthesia and surgery group, compared with the control group, one week after surgery. In addition, after immunostaining examination, an increase in Arc positive cells, which is a widely used nerve activity marker, was observed in the amygdala, and nerve activity was generated in the anesthesia and surgery group.

Free Research Field

集中治療、麻酔

Academic Significance and Societal Importance of the Research Achievements

心停止後に自己心拍が再開しても(1)脳損傷、(2)心筋障害、(3)全身性虚血再灌流障害、(4)残存する心停止の原病で構成される心停止後症候群により予後は依然厳しい。術後痛モデルマウスを用いた検討では、手術によって記憶の固定や想起が障害され、扁桃体の神経活動が活性化された。蘇生後脳症においても手術と同様に全身性の炎症反応が引き起こされると報告されており、その機序に扁桃体が関わっている可能性がある。