Investigation of anti-inflammatory effects of oxygen nanobubbles in a rat hydrochloric acid lung injury model

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18332
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 55060:Emergency medicine-related
• Research Institution: Fukushima Medical University
• Principal Investigator: Yoshida Keisuke 福島県立医科大学, 医学部, 助教 (00769573)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 急性呼吸窮迫症候群 / 酸素ナノバブル

Outline of Final Research Achievements

Aim: To investigate the anti-inflammatory effect of oxygen nanobubbles (ONBs) in an acute lung injury rat model. Materials & methods: In a rat hydrochloric acid lung injury model, ONB fluid was administered intravenously in the ONB group (n = 6) and normal saline was administered in the control group (n = 6). 4 h later, arterial partial pressure of oxygen (PaO2), mean arterial pressure and plasma inflammatory cytokines were measured. Results: There were no significant differences in the PaO2, mean arterial pressure or TNF-α and IL-6 levels between the two groups. Conclusions: No anti-inflammatory effect could be confirmed at the present ONB dose in the rat model of acute lung injury.

Free Research Field

麻酔科学、集中治療学

Academic Significance and Societal Importance of the Research Achievements

急性呼吸窮迫症候群は死亡率が高く、有効な治療法の確立が望まれている。近年注目されている酸素ナノバブルは、酸素含有量が高いことに加えて抗炎症作用を有しており、われわれは、酸素ナノバブルはARDSに対して有効であるという仮説を立てた。本研究の目的は、急性肺障害モデルラットに酸素ナノバブル水を経静脈投与し、動脈血酸素分圧・サイトカイン濃度の変化・肺の組織学的変化を経時的に観察することによって、酸素ナノバブルの経静脈投与がARDSの治療に有効か否かを実証することであり、酸素ナノバブル水経静脈投与の有効性・安全性が実証されれば、ARDSや他の肺疾患治療への応用も期待できると考えて研究を行った。