Research for mechanism of telomere lengthening in gliomas harboring ATRX mutation using patient-derived induced pluripotent stem cell

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18412
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Hokkaido University
• Principal Investigator: Ishi Yukitomo 北海道大学, 大学病院, 医員 (30812284)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 神経膠腫 / iPS細胞 / ATRX症候群

Outline of Final Research Achievements

We cultured 201B7, an iPS cell line established from healthy human, with feeder free condition using Neural Induction Medium as a preliminary experiment. After culturing, cells indicated positive staining of Nestin and PAX6 on fluorescent immunohistochemistry, which suggested sufficient induction of neural progenitor cells. To induce astrocytes from neural progenitor cells, we cultured these cells with condition of DMEM as basal medium and with various induction factors. However, we could not obtain sufficient amount of cells with GFAP positive cells. We will further study the culturing condition to induce astrocytes from neural progenitor cells, and will perform similar experiments using iPS cell derived from patient with ATRX syndrome.

Free Research Field

脳神経外科学

Academic Significance and Societal Importance of the Research Achievements

本研究では、予備実験として行った正常iPS細胞を用いたアストロサイトの分化誘導の段階で十分な誘導条件を確立できず、疾患特異的iPS細胞を用いた実験の段階まで進めることができなかった。よって目的としていたATRX症候群由来iPS細胞の分化誘導後の解析などは全く行えていない。より簡便かつ高い誘導効率をもったアストロサイトの分化誘導方法の確立が必要であり、今後の研究が望まれる。