Differentiation of the osteoblast and its role in the pathogenesis of the craniosynostosis

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K18431
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Saga University
• Principal Investigator: Yoshioka Fumitaka 佐賀大学, 医学部, 助教 (30620249)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 頭蓋縫合早期癒合症 / 骨芽細胞 / 間葉系幹細胞

Outline of Final Research Achievements

We were able to obtain bone specimens from four cases of syndromic craniosynostosis and three cases of nonsyndromic craniosynostosis throughout the study period.Although only a small number of bone specimens could be collected due to the spread of the infection of COVID-19, we attempted to identify the presence of mesenchymal stem cells immunohistochemically by immunostaining with a marker for mesenchymal stem cells.However, we had difficulty in immunostaining cultured cells and could not confirm whether the cultured cells contained mesenchymal stem cells.Therefore, we were not able to isolate and culture mesenchymal stem cells or analyze the genetic abnormalities and expression of Runx2 and Actin2, which we had planned to do afterwards, and we were not able to present our findings at a conference or in a scientific paper.

Free Research Field

小児脳神経外科学

Academic Significance and Societal Importance of the Research Achievements

研究期間全体を通して、症候群性頭蓋縫合早期癒合症4例、非症候群性頭蓋縫合早期癒合症3例の骨標本を採取することができた。この標本を用いて、現在も研究を継続中である。研究機関内には成果を上げることはできなかったが、得られた標本および培養細胞から、頭蓋縫合早期癒合症の病態解明に寄与する結果を導くべく、今後も研究を継続する予定である。