Establishment of Adipose Drived Stem Cell transplant administration method for cerebral infarction and elucidation of the role of the healthy side

2023 Fiscal Year Final Research Report

• Project/Area Number: 19K18432
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56010:Neurosurgery-related
• Research Institution: Nagasaki University
• Principal Investigator: Sadakata Eisaku 長崎大学, 医歯薬学総合研究科(医学系), 研究協力員 (10772139)
• Project Period (FY): 2019-04-01 – 2024-03-31
• Keywords: ADSC / cell therapy / Ischemic stroke

Outline of Final Research Achievements

An 80-minute transient middle cerebral artery occlusion model was created for SD rats. 24 hours later, ADSC or PBS was administered intraarterially. Rats were divided into three groups and evaluated: low dose group, high dose group, and control group. After administration, mNSS and body weight were measured to evaluate functional recovery, and brain tissue was collected 28 days after administration. Cresyl Violet staining was performed to evaluate the area of cerebral infarction.
Between the control group and the cell-administered group, significant functional recovery was observed in the cell-administered group. A significant reduction in the size of cerebral infarction nests was also observed in the cell-administered group.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

厚生労働省の国民生活基礎調査では脳血管疾患は脂肪率は第4位、要介護の直接原因は第1位となっている。このため脳梗塞患者に対して幹細胞投与による再生医療が進むことで寝たきりなどを予防し医療費の削減につながると考えられる。
本症例では細胞投与群で有意に脳梗塞層の縮小と機能予後の改善につながった。特に細胞投与群ではlow dose群でもhigh dose群と比較しても有意差なく機能予後改善が得られたため少量の自己脂肪細胞由来幹細胞投与で改善が得られることが示唆された。