Development of bone formation-promoting drugs based on osteoblast activation mechanism induced by carbon nanomaterials.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18495
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Shinshu University
• Principal Investigator: Ideta Hirokazu 信州大学, 医学部附属病院, 医員 (00838534)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 骨芽細胞 / カーボンナノホーン / カーボンナノマテリアル

Outline of Final Research Achievements

We have revealed that the osteogenesis-promoting effects of carbon nanohorns (CNH) are not mediated by membrane receptors on osteoblasts. Of the nanomaterials incorporated into cells, only CNH among carbon black, CNH, and nanoferrite showed stimulatory effects on osteogenic potential, suggesting that osteoblasts recognized the morphological characteristics of CNH intracellularly. Intracellular CNH were found to become vesiculated, with calcium phosphate accumulation seen around the vesicles. The precise relationship between this accumulation and the promotion of bone formation will be analyzed in future studies. In addition, since the evaluation of bone formation using the osteoblast system is affected by culture medium properties, it will be necessary to elucidate the culture conditions that reflect the bone formation reaction occurring in vivo.

Free Research Field

整形外科

Academic Significance and Societal Importance of the Research Achievements

カーボンナノマテリアル（CNM）による骨形成促進効果のメカニズムはこれまで不明のまま、ドラッグデリバリーシステムやインプラントなどへの応用研究が進められてきた。本研究によってCNMの骨形成能促進効果を期待した医療開発の場合、細胞内に取り込まれることを前提にして開発すべきであり、用途を明確にすることができた。さらにCNH周辺で観察されたリン酸化カルシウムの沈着は未解決である基質小胞形成仮説におけるメカニズム解明の一助になると考えている。