Novel treatment strategy for iatrogenic nerve root injury using adipose-derived stem cell

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18538
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: Osaka City University
• Principal Investigator: Takahashi Shinji 大阪市立大学, 大学院医学研究科, 病院講師 (80735605)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 脊椎 / 手術 / 脂肪由来幹細胞 / 熱損傷 / 癒着

Outline of Final Research Achievements

One of the causes of poor improvement in spine surgery is nerve damage due to adhesions around the dura mater or heat injury. In this study, we used animals to model each of these factors and took measures to prevent them. Cooling during surgery, steroids, and undifferentiated adipose stem cells (uADSCs) were tried. In the thermal injury model, cooling water and uADSCs were effective in preventing demyelination. There was no clear effect of uADSC on peridural adhesions. The results of this study suggest that uADSC is an effective treatment for thermal injury, but further study is needed for peri-dural adhesions.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

脊椎手術では術後に症状が増悪する例や改善が乏しい例が散見される。その要因は多岐にわたるが一つとして熱損傷や神経周囲の癒着が考えられる。本研究ではその予防策を検討するために行われた。脂肪由来幹細胞は採取が容易で再生医療によく用いられる。本研究でも脂肪由来幹細胞による神経の脱髄予防が示唆された。しかし脂肪由来幹細胞は採取から培養まで1カ月以上要するため、今後は術後症状が増悪する例に対して使用可能かさらなる検討が必要である。