Cell and Growth factor deliverly using in situ formed hydrogel for treatment of bone defect

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18546
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56020:Orthopedics-related
• Research Institution: 湘南医療大学
• Principal Investigator: Sekiguchi Hiroyuki 湘南医療大学, 臨床医学研究所, 研究員 (90547233)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 骨形成蛋白 / 徐放 / ゲル剤

Outline of Final Research Achievements

We examined the effectiveness of an in situ-formed hydrogels (IFHGs) made of hyaluronan (IFHG-HA) containing BMP2 for promoting bone formation in a mouse critical size bone defect model. C57/BL6J mice received a 2-mm femoral critical-sized bone defect before being randomly assigned to one of the following treatment groups (n=6): control (no treatment), IFHG-HA only, PBS with BMP2, and IFHG-HA with BMP2. X-ray radiographs were utilized to track new bone formation, and micro-computed tomography and histological examination were performed on new bone formed at the bone defect site two weeks after surgery. Mice treated with PBS with BMP2 and IFHG-HA with BMP2 had greater bone volume (BV) and bone mineral content (BMC) than those receiving control, and successfully achieved consolidation. Mice treated with IFHG-HA with BMP2 had significantly higher BV and BMC than those treated with PBS with BMP2. IFHG-HA may be an effective carrier for BMP2 to enable delivery for bone defect repair.

Free Research Field

整形外科学

Academic Significance and Societal Importance of the Research Achievements

外傷や腫瘍掻爬後の広範囲骨欠損は治療に難渋する。治療の長期化に伴う患者の社会復帰の遅延は、患者に肉体的・精神的苦痛を与えるとともに医療費の増加と社会経済的損失に直結する。本研究で開発したゲル剤は広範囲骨欠損治療に重要であると考えられる。