development of new assay for detecting circulating prostate cancer cell using new measuring system for circulating tumor cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K18563
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56030:Urology-related
• Research Institution: Kagawa University
• Principal Investigator: Kato Takuma 香川大学, 医学部, 学内講師 (70625673)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 前立腺癌 / 循環腫瘍細胞

Outline of Final Research Achievements

We have developed a technique for moderately controlling hydrophilicity on plastic substrates by surface treatment with oxygen plasma. When a solution of cultured prostate cancer cells added to pseudo-blood was sprinkled on the substrate, it was confirmed that the cells adhered to the surface of the substrate in a single layer. When cells are piled up, tumor cells are mixed in with blood cells, making it difficult to identify tumor cells, but with this technique, tumor cells can be easily identified. We plan to conduct clinical studies to verify the feasibility of the clinical application of this experimental system.

Free Research Field

前立腺癌

Academic Significance and Societal Importance of the Research Achievements

前立腺癌の予後および治療効果判定のマーカーとして循環腫瘍細胞（Circulating Tumor Cells; 以下CTC）は近年注目を集めています。その一方で、CTCの解析は高額の費用が必要で、解析に長時間を要するため、一般臨床では普及していません。本研究で開発された手法は手技が簡便で、高額な検査機器は用いません。このCTCの解析方法が通常の診察に用いることができるようになれば、手術や放射線治療、抗がん剤治療などの治療後の再発をより早い段階で発見できるようになります。