The presense of HPV virus in Surgical Smoke of Cervical conization

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18655
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Kansai Medical University
• Principal Investigator: HISAMATSU Yoji 関西医科大学, 医学部, 講師 (20465644)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: HPV / サージカルスモーク

Outline of Final Research Achievements

The presence of HPV DNA was shown in the surgical smoke (SS) generated by cervical conization and in the filtered gas of the surgical mask. This result was not published in previous studies at this time. In addition, since there was no previous study on the infectivity of HPV in SS, skin epidermal keratinized cells were exposed to variously treated specimens and the expression of the HPV E1 ^ 4 region was examined, but the amount of virus was very low. Therefore, it was judged that the sensitivity was low and the infection assay could not be established, and it was impossible to give novelty. Therefore, for the above-mentioned quantification of viral DNA, we created a DNA standard in which HPV 16/18/52/58 type E6 region + human β-globin was cloned, and for cervical lesions, which is another project in our department. I am thinking of applying it to the judgment of therapeutic effect.

Free Research Field

産科学婦人科学

Academic Significance and Societal Importance of the Research Achievements

サージカルスモークに含まれるウイルスの感染性に関しては医療従事者の産業衛生の分野で懸念はされていたが、特に2020年以降新型コロナウイルスのパンデミックにより、医療従事者内での認知が進んできた印象である。HPV感染により子宮頸部異形成は発生し、その病変を術者の顔面近くで操作する円錐切除術ではスモークの曝露のリスクが高い。HPVの存在証明は先行研究で行われていたが、今回は確立できなかったものの存在のみならず感染性に着目し研究を行った。医療従事者の産業衛生の面でも示唆に富む研究であり、今後の別の研究にも応用可能な検査方法を確立できつつある。