Establishment of an in vitro implantation model with embryo organoid

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18685
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56040:Obstetrics and gynecology-related
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Takaya Abe 国立研究開発法人理化学研究所, 生命機能科学研究センター, 技師 (10720609)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: マウス / 着床 / 胚培養 / 子宮

Outline of Final Research Achievements

An in vitro implantation model which can recapitulate the cell-cell interaction between an embryo and a uterine tissue is required to make better understanding of the implantation mechanism. However, it has not been developed yet. To overcome this issue, a mouse in vitro implantation model has been tried to be developed by using 3-dimensional cell culture of endometrial tissue and embryo culture. As a result, the embryo culture during preiimprantation stage from blastocyst (E3.5) to around E5.5 stage was achieved and a 3D culture method of uterine tissue using endometrial epithelial cells and stromal cells was succeeded to be established.

Free Research Field

マウス初期発生

Academic Significance and Societal Importance of the Research Achievements

試験管内で子宮組織の細胞環境を再現して、そこで胚を発生させる着床モデルの開発は、胚発生における子宮の役割、着床や胎盤形成のメカニズムを解明する上で重要な役割を果たす可能性があり学術的な意義は大きい。