Comprehensive study on the pathogenesis of endolymphatic hydrops in Meniere's disease

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18750
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56050:Otorhinolaryngology-related
• Research Institution: Kindai University
• Principal Investigator: SHIRAISHI KO 近畿大学, 医学部, 非常勤講師 (90775660)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 内リンパ嚢 / タンパク質 / プロテオーム解析 / イオンチャネル / メニエール病 / 聴神経腫瘍

Outline of Final Research Achievements

The endolymphatic sac tissue of Meniere's disease patients and acoustic neuroma patients was surgically collected, and the proteins contained in the endolymphatic sac were comprehensively quantitatively analyzed using the proteomics analysis method. When 5 cases in each group were analyzed, 2738 proteins could be identified and 2677 proteins could be quantified. Of these, 17 proteins were detected only in the Meniere's disease group and 146 were detected only in the target group. As a result of quantitative analysis of 2514 proteins detected in both groups, 10 proteins had a significantly increased expression level and 10 proteins had a significantly decreased expression level in the Meniere's disease group. Next, as a result of annotating all proteins, a total of 19 ion channels could be identified.

Free Research Field

耳鼻咽喉科　内耳

Academic Significance and Societal Importance of the Research Achievements

メニエール病の原因と言われている内リンパ水腫を引き起こす病態を見つけるすことがこの研究の目的である。内リンパ水腫を引き起こす原因と言われている内リンパ嚢の組織をメニエール病患者と対照群患者で比較することでメニエール病患者特有のタンパク質を見つけることで内リンパ水腫の発生器所を解明していく。