2022 Fiscal Year Final Research Report
Elucidation of the mechanism underlying planar cell polarity regulation in inner ear hair cells by ICK ciliary kinase
Project/Area Number |
19K18780
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 56050:Otorhinolaryngology-related
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Research Institution | Institute of Physical and Chemical Research (2021-2022) Kyoto University (2020) Kyoto Prefectural University of Medicine (2019) |
Principal Investigator |
Okamoto Shio 国立研究開発法人理化学研究所, 脳神経科学研究センター, 研究員 (10838139)
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Project Period (FY) |
2019-04-01 – 2023-03-31
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Keywords | 蝸牛有毛細胞 / 運命決定 / 接着斑 |
Outline of Final Research Achievements |
The cochlea, a hearing organ, has a unique spiral structure which makes it possible for us to hear various sound frequency. This structure is formed by convergent extension (CE) that is dynamic cell arrangement. Another structure important for elaborate hearing is planar cell polarity (PCP) which is the U- or V-shaped staircase structure formed by actin protrusion called stereocilia on the apical surface of hair cells. CE and PCP formation in hair cells are regulated by cilia on the apical surface of hair cells in cochlear development, whereas the mechanism these two have in common remains to be explored. To elucidate this mechanism, we forcused on the component of focal adhesion between cells and substrates because one of the focal adhesion components were expressed in both apical and basal membranes of progenitor cells. Unexpectedly one of the focal adhesion components we focused on affected cell fate specification, while they affected neither CE nor PCP in hair cells.
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Free Research Field |
蝸牛有毛細胞発生
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Academic Significance and Societal Importance of the Research Achievements |
近年、細胞外からの機械的刺激が細胞の増殖、分化、移動などを制御することが知られているが、蝸牛の発生における機械的刺激の役割については、その多くが未解明である。本研究は、細胞-基質間に存在する接着斑を介した機械的刺激が、聴覚受容に必須である有毛細胞の運命決定に重要な役割を果たす可能性を明らかにした点で学術的意義がある。また今後、機械的刺激が有毛細胞の運命決定を制御するメカニズムについて明らかにすることにより、現時点では未解明である、有毛細胞障害により一旦失われた聴覚を、意思疎通が可能となるレベルに回復させる手法の解明に大きく貢献できる可能性を秘めており、社会学的にも意義があると考えられる。
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