2019 Fiscal Year Research-status Report
Pathophysiology and novel drug development targeting deafness-associated potassium channel KCNQ4
| Project/Area Number |
19K18802
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| Research Institution | Shinshu University |
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Principal Investigator |
デイ ティモシー 信州大学, 医学部, 研究員 (00838667)
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | KCNQ4 / model mice / hearing loss |
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| Outline of Annual Research Achievements |
Disabling hearing loss is one of the most frequent disorders that affects 5% of the world’s population, and half of congenital hearing loss is caused genetically. KCNQ4 encodes for a potassium channel which maintains potassium concentrations surrounding the hair cells, the sensory cells in the inner ear. Dysregulation in potassium concentrations gradually damages the hair cells, resulting in hearing loss.
In this year, we quantitatively measure the auditory phenotype of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE). Morphology is verified by scanning electron microscope and transmission electron microscope in addition to fluorescent immunostaining also starting.
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| Current Status of Research Progress |
Current Status of Research Progress
2: Research has progressed on the whole more than it was originally planned.
Reason
We can quantitatively measure the auditory phenotype of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE) .
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| Strategy for Future Research Activity |
We will also perform the auditory measurement of newly developed KCNQ4 mutation knock in model mice, objectively using the auditory brain stem response (ABR) and otoacoustic radiation (OAE). We will also perform in vitro experiment for phenotypic analysis of KCNQ4 mutation.
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| Causes of Carryover |
当初計画していたのと比較しマウスの繁殖が悪く、形態の観察が次年度以降になり観察のための試薬代金が繰越しとなった。現在、順調に繁殖するようになったため次年度以降に研究を実施する
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Research Products
(5 results)
