2020 Fiscal Year Annual Research Report
Pathophysiology and novel drug development targeting deafness-associated potassium channel KCNQ4
| Project/Area Number |
19K18802
|
|---|
| Research Institution | Shinshu University |
|---|
Principal Investigator |
デイ ティモシー 信州大学, 医学部, 研究員 (00838667)
|
|---|
| Project Period (FY) |
2019-04-01 – 2021-03-31
|
| Keywords | KCNQ4 / inner ear / hair cell |
|---|
| Outline of Annual Research Achievements |
We have completed experimental testing for data in the project titled “Pathophysiology and novel drug development targeting deafness-associated potassium channel KCNQ4”. Using the KCNQ4 c.211delC knock-in mouse we collected all auditory brain stem response (ABR), and otoacoustic radiation (DPOAE). The KCNQ4 c.211delC mice had significant hearing loss compared to wild-type control and heterozygous control mice. Hearing loss began shortly after birth, and the mice were deaf by 2 months old. DPOAE results indicate loss of outer hair cells (OHC). Inner ear cellular composition was examined by confocal microscopy to visualize loss of OHC.
The project is currently in manuscript preparation for publication in a peer reviewed journal.
|
