2021 Fiscal Year Final Research Report
Otolith function in P2X2 receptor knockout mice using a linear acceleration stimulator.
| Project/Area Number |
19K18805
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 56050:Otorhinolaryngology-related
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| Research Institution | Osaka University |
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Principal Investigator |
Harada Shotaro 大阪大学, 医学部附属病院, 医員 (10824740)
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| Project Period (FY) |
2019-04-01 – 2022-03-31
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| Keywords | 前庭機能 / P2X2受容体 / 内耳 |
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| Outline of Final Research Achievements |
The P2X2 receptor is a cation-permeable ion channel-embedded receptor located in the inner ear and is involved in the regulation of potassium ion, a composition ion of the endolymphatic cavity of the inner ear. Our laboratory has KO mice for P2X2 receptors, and we evaluated vestibular function in these mice. Since previous studies have confirmed that the semicircular canal function is impaired, in this experiment, we evaluated the function of the otolith, the other vestibular organ that is present.
No significant difference in otolith oculomotor reflex was observed between P2X2KO, WT mice.These results suggest that P2X2 receptors affect semicircular canal function but not otolith function.
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| Free Research Field |
内耳平衡機能
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| Academic Significance and Societal Importance of the Research Achievements |
P2X2受容体の遺伝子変異は、ヒトでは常染色体優性遺伝の形式をとり、10から20歳代までに中から高音域を中心とした進行性の感音難聴を認め、前庭機能には異常を認めないとされている。
P2X2受容体は、陽イオン透過性イオンチャネル内蔵型受容体であり、内耳内のKイオン濃度を調整し、内耳では蝸牛の有毛細胞、ラセン神経節、支持細胞や外有毛細胞の不動毛に存在することが報告されている。以前の当研究室の研究では、P2X2受容体KOマウスでは、半規管機能の低下を認めていた。今回、耳石器機能の評価を行ったが、耳石器機能の異常は認めなかった。
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