Establishment of gene therapy using b-FGF gene-loaded Sendai virus for chronic wounds.

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K18904
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Chiba University
• Principal Investigator: Ogata Hideyuki 千葉大学, 医学部附属病院, 助教 (60646024)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 慢性創傷 / Werner症候群 / リンパ管 / 老化

Outline of Final Research Achievements

In preparation for considering the use of b-FGF-transfected Sendai virus for chronic wounds, it was necessary to elucidate the mechanism of why chronic wounds occur. Werner syndrome (WS) is a disease with a high probability of developing intractable skin ulcers and is useful as a model for understanding the factors and mechanisms involved in the development and progression of skin ulcers. In this study, we first performed a histopathological exploration of the ulcer site in WS patients. The results revealed that the calcification under the skin of the ulcer was located in the lymphatic vessels. They also used immunochemical staining to show the accumulation of abnormal WRN protein in lymphatic endothelial cells, suggesting that the accumulation of calcification may be caused by aging of lymphatic vessels.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

本研究はWS患者の慢性創傷においてリンパ管の老化現象に似た異常が生じていることを明らかにした。このことはWS患者の創傷治癒遅延のメカニズムの解明に寄与する可能性がある。また、WSではない一般的な難治性創傷においても、リンパ管の異常が関係している可能性があり、本研究の成果がこれらの現象が創傷治癒に及ぼす影響を評価するための指標や手法を提供するものと考える。また当初の目的であった、慢性創傷に対するb-FGF導入センダイウイルスの使用においてもその効果の機序の解明や評価方法に寄与するものであると考えられる。