The development of a manufacturing device for Micronized Cellular Adipose Matrix and its application in regenerative therapy

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18911
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Jichi Medical University
• Principal Investigator: Morita Yuhei 自治医科大学, 医学部, 臨床助教 (90834474)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 脂肪幹細胞 / 微小細片化組織 / MCAM / 再生医療 / 脂肪細断

Outline of Final Research Achievements

The present study discussed the optimal method for processing the micronized cellular adipose matrix (MCAM) which is riched in adipose-derived stem cells (ASCs). Furthermore, we investigated whether a prophylactic administration of products containing adipose tissue-derived stem cells immediately after radiotherapy could prevent the development of long-term functional disorders in irradiated tissues.
Our results showed that the wound healing was significantly delayed by a total 40-Gy dose of irradiation in mice. And the prophylactic treatments with three different types of human adipose tissue-derived products significantly prevented the radiation induced histological changes and accelerated wound healing compared with the vehicle-treated irradiated group. Such prophylactic treatments have the potential to improve wound healing of irradiated tissue and clinical outcomes of reconstructive surgery required after cancer radiotherapy.

Free Research Field

幹細胞の臨床応用

Academic Significance and Societal Importance of the Research Achievements

MCAMは、これまでの脂肪移植とは全く異なるアプローチであり、組織の賦活化、肥沃化、抗炎症など組織の機能不全の治療に照準を合わせ、ASCの効能を利用しつつも、規制や安全性の問題を避けて、容易に速やかに完了できる手技であり、学術的にも医学的にも期待される新規性の高い治療アプローチである。本研究は、これまでの研究成果を、臨床に橋渡しして実現するための発展性、創造性の高い研究となる。