In vitro wound healing dynamics using Fucci

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18915
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Keio University
• Principal Investigator: YABUKI Hanayo 慶應義塾大学, 医学部(信濃町), 助教 (70793305)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 創傷治癒

Outline of Final Research Achievements

Based on data on cell division and migration in an in vitro tissue culture system, factors previously reported to promote cell division and cell migration and promote wound healing were mixed into the tissue culture medium. Tissue culture was performed and the effect on wound healing was observed to investigate whether the part where cell division occurred was activated or the part which was not divided by normal wound healing was activated. In the study using the cell culture system, we also observed in real time how each influences cell division and migration using a time-lapse fluorescence microscope.

Free Research Field

形成外科

Academic Significance and Societal Importance of the Research Achievements

これまで、リアルタイムで細胞の分裂が不明であった創傷治癒過程の細胞の動態を、生体を模擬した形で観察できた。このことから、今後の細胞レベルでの創傷治療薬の開発や、スクリーニングに有用であると考えられた。