Exogenous Stimulation Effectiveness Verification of Adipose Tissue-Derived Stem Cells from healthy people and Diabetic Patients for Wound Care Application

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K18935
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 56070:Plastic and reconstructive surgery-related
• Research Institution: Juntendo University
• Principal Investigator: Matsui Chihiro 順天堂大学, 医学部, 非常勤助手 (80815019)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 脂肪組織由来幹細胞 / サイトカイン / パラクライン効果 / リンパ管新生 / 血管新生

Outline of Final Research Achievements

In this study, the characteristics of the cytokine-releasing effect (paracrine effect) of adipose-derived stem cells (ADSCs) were examined in detail in order to effectively apply them to therapeutic use. In the first step, we administered b-FGF, which is known to enhance the paracrine effect of ADSCs, and verified by RT-PCR a total of 181 cytokines released in the preliminary stage of autocrine effect. In the second step, we used ADSCs of various ages, genders, and races to confirm by RT-PCR whether b-FGF stimulation caused the release of CXCL1 and IL8, which proved that the release of CXCL1 and IL8 could be confirmed regardless of race, age, or gender. The same experiment was also performed with ADSCs collected from diabetic patients and the same response was obtained.In the third step, to investigate the effect of CXCL1 and IL8 on wound healing, we conducted a validation on angiogenesis and lymphangiogenesis and found that both were promoted.

Free Research Field

脂肪組織由来幹細胞

Academic Significance and Societal Importance of the Research Achievements

ADSCsを用いた治療についてはこれまで多くの論文が報告されており、その効果をｂ－FGFが促進することも知られてきたが、これまで「何の成分が効果的であるから治療に役立つ可能性があるのか」が不明瞭なままだった。またこれまでADSCsをb-FGFで刺激した際に得られる反応に対し、創傷治癒に関連する１８１種類もの遺伝子に関しての検証は行われておらず、詳細な検証が行われたのは本研究が初めてとなる。そのため漠然と役立つという形で定義されていたADSCs治療に関し、具体的に創傷治癒に効果のあるサイトカインを同定したことは、今後の再生医療の発展に大きく寄与すると考えられる。