The development of dentin formation-promoting peptides using odontoblast-specific Fam20C overexpression mice

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K18944
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Osaka University
• Principal Investigator: Hirose Katsutoshi 大阪大学, 歯学研究科, 助教 (00824898)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 象牙質 / Fam20C / リン酸化 / Dmp1 / DSPP / DPP / 酸性リン蛋白質

Outline of Final Research Achievements

Family with sequence similarity 20, member C (FAM20C) phosphorylates secretory proteins at S-x-E/pS motifs, and is highly expressed in mineralized tissues, implying the role of FAM20C-mediated phosphorylation in their homeostasis. The role of odontoblastic FAM20C-mediated phosphorylation in tooth dentin remains unclear. Here, we investigated the role of FAM20C in dentin formation using odontoblast-specific Fam20C overexpression (Fam20C-Tg) mice, which showed no alterations in serum calcium and phosphate levels.
This study revealed that FAM20C-mediated phosphorylation regulates dentin matrix production and mineralization and odontoblast differentiation.

Free Research Field

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Academic Significance and Societal Importance of the Research Achievements

本研究により、生体硬組織に特異的かつ豊富に存在する酸性リン蛋白質のリン酸化は、象牙質の石灰化の促進に働くことが明らかとなった。「リン酸化」は、暫間的間接覆髄法 (IPC法)での感染歯質の再石灰化、直接歯髄覆髄法での新規硬組織誘導、象牙質知覚過敏症や初期う蝕の再石灰化療法など歯科治療の新規治療ターゲットとなる可能性が示唆された。