Functional analysis of monocarboxylate transporter 1 in bone and bone marrow adipose tissues

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18950
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57010:Oral biological science-related
• Research Institution: Showa University
• Principal Investigator: SASA KIYOHITO 昭和大学, 歯学部, 助教 (50823069)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 脂肪細胞 / 分化 / MCT

Outline of Final Research Achievements

In aging and obesity, it is known that the differentiation of pluripotent mesenchymal stem cells into adipocytes becomes predominant over the differentiation into osteoblasts, which is one of the causes of bone weakening. Adipose tissue is characterized by increased lactic acid production due to decreased mitochondrial function, and is considered to be closely related to the monocarboxylic acid transporter (MCT). In this study, we investigated how MCT affects the differentiation into osteoblasts and adipocytes. Mct1 siRNA was introduced into cells and gene knockdown was performed to confirm promotion of adipocyte differentiation and suppression of osteoblast differentiation. The function of MCT1 during adipocyte differentiation was comprehensively examined by metabolome analysis for changes in metabolites.

Free Research Field

骨代謝調節

Academic Significance and Societal Importance of the Research Achievements

本研究は骨および骨髄脂肪組織におけるMCT1の機能を解析することである。MCT1の機能は、癌細胞、神経および筋肉などの糖代謝が盛んな組織で広く研究されているが、骨組織および脂肪組織におけるMCT1の役割に着目した研究はほとんど報告されておらず、本研究は極めて独創的であると考えられる。また、MCT1は分化の振り分けを介して、骨組織および骨髄脂肪組織の形成に関与する可能性が高いことから骨粗鬆症の新たな治療標的分子となる可能性があり、臨床的にも重要な意義を持つと考えられる。