The study of periodontal regeneration using growth factor delivery system due to collagen anchoring

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K18997
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Okayama University
• Principal Investigator: Nakamura Shin 岡山大学, 大学病院, 医員 (70837690)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: ドラッグデリバリーシステム / 成長因子 / 歯周組織再生 / コラーゲン

Outline of Final Research Achievements

Basic fibroblast growth factor (bFGF) has been utilized for periodontal regeneration. However, its application is limited because of its low target specificity and local retention. Therefore, we focused on CBFGF consisted of bFGF and collagen-binding domain (CBD) to promote regenerative efficacy. The aim of this study is to evaluate the function of CBFGF in detail and to expand the versatile application of CBD. Retention of CB-bFGF in local tissue and mesenchymal stem-like cells in periodontal tissue was evaluated in rats. Consequently, we showed CBFGF exerted the prolonged efficacy due to the improvement of local retention. Moreover, we tried to connect bFGF and CBD using chemical cross-linking agent. Molecular weight of cross-linked CBFGF was bigger than recombinant fused CBFGF. It is suggested that multiple bFGFs bind to CBD, or bFGFs formed complex with each other. Further investigation is necessary to examine the ratio and efficiency of cross-linked reaction.

Free Research Field

歯科保存治療学・歯周病学

Academic Significance and Societal Importance of the Research Achievements

bFGF製剤は，局所滞留性や標的特異性に乏しいため，歯周組織再生療法において，適応症が垂直性骨吸収へ限定されている。CBFGFは，bFGFの欠点を克服することによって，水平性骨吸収に対する歯周組織再生療法への応用に加えて，先天性骨欠損や顎堤増大術への応用に寄与できる可能性がある。また，CBDとタンパク質の化学架橋によって，新規の薬剤デリバリーシステムとして，bFGFだけでなく他の生理活性物質への連結など，CBDの汎用性の向上が期待できる。