Effect of Bone Resorption Inhibitor on Inflammatory Bone Destruction Using Periodontal Disease Model Mouse

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19035
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57030:Conservative dentistry-related
• Research Institution: Showa University
• Principal Investigator: MIku Kuritani 昭和大学, 歯学部, 兼任講師 (00826737)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 歯槽骨破壊 / 歯周病 / 骨代謝 / 骨吸収抑制薬

Outline of Final Research Achievements

The handicapped are more likely to develop periodontal disease because it is difficult for them to manage their oral cavity by themselves due to trunk disorders of the limbs.
In this study, we analyzed the effects of administration of anti-RANKL antibody and bisphosphonate (BP), which is a bone resorption inhibitor, preparation on inflammatory bone destruction.
Results of ligating silk thread to the maxillary second molar of an 8-week-old male mouse to create an inflammatory periodontal disease model. In the mice to which the anti-mouse RANKL antibody (OYC1; 5 mg / kg) was continuously administered at 1-week intervals for 4 consecutive weeks, the degree of recovery from the microCT image was mild. In the bone defect model in which the mouse femur was pierced through the bone marrow with a dental bar, the degree of bone repair was low in the anti-RANKL antibody-administered group, but bone repair was promoted in the zoledronic acid-administered group.

Free Research Field

障害者歯科

Academic Significance and Societal Importance of the Research Achievements

障害者は口腔清掃の重要性が理解できないことや四肢体幹障害などにより、自己での口腔管理が困難なため歯周病を発症しやすく、炎症性歯槽骨破壊のリスクが非常に高い。従って、物理的なプラークコントロールでなく、化学的に歯槽骨破壊を抑制する薬物療法の開発は重要課題の１つである。マウス臼歯に絹糸を結紮することで細菌感染に起因する炎症性骨破壊モデルに対する薬剤投与の効果を解析することは将来的な臨床応用への足がかりとなる。