2020 Fiscal Year Final Research Report
Generating salivary gland cells from mouse embryonic fibroblasts using the direct reprogramming technique
Project/Area Number |
19K19057
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 57040:Regenerative dentistry and dental engineering-related
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Research Institution | Showa University |
Principal Investigator |
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | 唾液腺発生 / ダイレクトリプログラミング |
Outline of Final Research Achievements |
The aim of this project is to generate salivary gland cells from mouse embryonic fibroblasts (MEF) using the direct reprogramming technique. Four transcription factors (TP63/TFAP2a/ cMYC/GRHL2) were co-transducted into MEF, and these MEF were grown in air-liquid interface culture. They formed a CK13-positive stratified epithelium (MEF-derived oral mucosal epithelial-like tissue). In addition, Six transcription factors (TP63/TFAP2a/ cMYC/GRHL2/Sox9/Foxc1) were co-transducted into MEF. They formed CK18, AQP5-positive epithelial cells (MEF-derived glandular epithelial-like cells) in monolayer culture conditions. We plan to continue the examination of the salivary gland differentiation and maturation methods from MEF-derived oral mucosal epithelial-like tissues or MEF-derived glandular epithelial-like cells.
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Free Research Field |
口腔病理学
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Academic Significance and Societal Importance of the Research Achievements |
本研究課題によって唾液腺再生技術開発に向けての基盤的なデータを得ることができた。MEFは細胞数の確保が容易であり、ダイレクトリプログラミングは腫瘍化のリスクが低い点から、ダイレクトリプログラミングによるMEFから唾液腺の作出はより安全で有効な唾液腺再生技術となるものと期待される。本研究成果をヒト唾液腺再生、そして口腔乾燥症の根本的治療法へ応用することにより、口腔内環境のみならず全身のQOLの維持・増進をもたらすものと期待される。
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