2020 Fiscal Year Final Research Report
The novel iPSCs differentiation method toward tooth organ by applying the Organ-on-a-Chip technology
| Project/Area Number |
19K19116
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| Research Category |
Grant-in-Aid for Early-Career Scientists
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| Allocation Type | Multi-year Fund |
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| Review Section |
Basic Section 57050:Prosthodontics-related
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| Research Institution | Tohoku University |
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Principal Investigator |
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| Project Period (FY) |
2019-04-01 – 2021-03-31
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| Keywords | 歯の再生 / iPS細胞 / 分化誘導 |
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| Outline of Final Research Achievements |
In this term for the research, the optimization of the method for human iPS cells (hiPSCs) differentiate towards the dental epithelial cells has performed. After hiPSCs were adapted in the feeder-free environment, cultured in the dental epithelial cell differentiation medium which contained the BMP-4 (BMP-4 had reported as the essential factor to form the tooth germ) and the Lithium Chloride (LiCl; Wnt/beta-Catenin signal activating factor).
I prepared the differentiation medium has been added such factors in several concentration manners and cultured the hiPSCs in different component medium. Finally, gene expression analysis of the cells has been conducted in some differentiation stages.
According that, the optimized condition has been discovered for hiPSC's dental epitherial differentiation.
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| Free Research Field |
外科系歯学
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| Academic Significance and Societal Importance of the Research Achievements |
本研究成果として，フィーダーフリー環境に馴化したヒト由来iPS細胞を対象とした，歯胚上皮細胞への分化誘導法の最適化に関する所見が得られた。同所見は，iPS細胞からエナメル芽細胞等，歯胚上皮系の細胞・組織を樹立する方法を確立する一助となり，発生学的な研究への応用や，齲蝕治療等において既存の手法を更に発展させる，技術革新に応用されることが期待できる。
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