Analysis of Annexin A8 in EMT-mediated Oral Cancer Infiltration

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19161
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Hiroshima University
• Principal Investigator: Ishida Fumi (田中扶美) 広島大学, 医系科学研究科(歯), 専門研究員 (60625979)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: EMT / Partial EMT / Snail / Anexin A

Outline of Final Research Achievements

I established an experimental system to reproduce partial EMT (pEMT) and EMT in stages by introducing Snail into an oral squamous cell carcinoma cell-line. The expression of Annexin A8, which was initially the subject of research in this project, could not be detected by the analysis using RNAseq conducted in the final year of this project. However, it was found that Annexin A10 was disappeared by pEMT and was upregulated by EMT, Annexin A6 was gradually upregulated from pEMT to EMT, and Annexin A9 was upregulated by pEMT and EMT.

Free Research Field

口腔外科学

Academic Significance and Societal Importance of the Research Achievements

口腔扁平上皮癌にSnailを導入し、パーシャルEMT（ｐEMT）とEMTを段階的に再現する実験系を確立し、RNAseqにより全遺伝子の発現をトレース可能となった。膨大な遺伝子発現プロファイルを、公開データベースと統合解析することで単一遺伝子Snailの導入がｐEMTにとどまり、いかにEMTへと移行するのかを突き止める足掛かりとなった。またEMTをpEMTへ逆戻りする実験系を開発できたが、核内受容体の遺伝子発現が要であることが判明し、そのリガンドが癌悪性度を制御できる可能性を発見した。