Induction culture of osteoblast differentiation for amniotic membrane derived mesenchymal stem cells focusing on cell adhesion mechanisms

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19168
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Nagasaki University
• Principal Investigator: Egashira Kazuhiro 長崎大学, 病院(歯学系), 助教 (50638096)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: 骨再生 / 間葉系幹細胞 / 周産期産物 / 同種

Outline of Final Research Achievements

Human umbilical cord (UC) -MSCs and AM-MSCs have a higher proliferative potential, however osteoblast differentiation ability is quite limited when compared to bone marrow-MSCs (BM-MSCs). Therefore, these MSCs usually require the long-term culture for differentiation but even differentiated cells cannot show sufficient in vivo osteo-inducibility. In this study, when examined various 2D and 3D matrix cultures on AM-MSCs, we found a culture with some inhibitors of actin-polymerization, which were derived from marine products, promotes the osteoblast differentiation and in vivo osteo-inducibility. However, different feature was recognized on osteoblastic differentiation among UC- and AM-MSCs, and the osteoblast differentiation ability of AM-MSCs was still limited compared to that of UC-MSCs. We are currently investigating the optimal 3D culture condition of osteoblast differentiation for AM-MSCs focusing on dynamics of actin cytoskeleton.

Free Research Field

再生医療

Academic Significance and Societal Importance of the Research Achievements

顎骨腫瘍や口蓋裂顎裂などによる顎骨・歯槽骨欠損を対象とした骨再生治療に応用する同種骨芽細胞製品の開発研究については、組織採取の侵襲がなく、安定供給と大量生産が可能である周産期産物由来MSCによる細胞製品が開発できれば、即納性と有効性が確保された治療を提供できるようになる。その結果、適切な咀嚼機能の回復がより簡便になされるようになれば、健康寿命の延伸にも大きく貢献できると考える。