Efficient Novel Cleft Lip and Palate Treatment Strategy Using Human Mesenchymal Stem Cells

2022 Fiscal Year Final Research Report

• Project/Area Number: 19K19231
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Tottori University
• Principal Investigator: NARAI Takashi 鳥取大学, 医学部, 助教 (40569266)
• Project Period (FY): 2019-04-01 – 2023-03-31
• Keywords: 骨芽細胞 / ヒト間葉系幹細胞 / 口唇口蓋裂

Outline of Final Research Achievements

The aim of this study was to create a less invasive and more reliable treatment strategy for cleft lip and palate by efficiently osteogenic differentiating human mesenchymal stem cells and transplanting the cells into the jawbone defect site.
The purified osteoblasts established an osteogenic differentiation gene profile at least one week earlier than the unpurified osteodifferentiated cells, and the linkage was confirmed. Continued induction of osteogenic differentiation also enhanced mineralization. These results suggest that osteoblast populations with improved heterogeneity may establish favorable conditions for bone regeneration.

Free Research Field

口腔顎顔面外科学

Academic Significance and Societal Importance of the Research Achievements

ヒト間葉系幹細胞を利用した骨再生医療は以前より施行されていたが、骨分化誘導効率や治療結果の確実性が障壁となっており未だ普及しているとは言い難い状況である。本研究によりこれらの問題点の解決策の一つとして患者自身の間葉系幹細胞を骨芽細胞へ分化誘導後に骨芽細胞を純化し移植する細胞治療の可能性が示された。この方法は純化した骨芽細胞集団を治療に応用することにより、骨再生に有利な条件が構築され従来の方法と比較して効率的で確実性のある骨再生法の創出が期待される。