Hard tissue regeneration using transplantation of adipose tissue-derived regenerative cells

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K19244
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 57060:Surgical dentistry-related
• Research Institution: Showa University
• Principal Investigator: Masakatsu Itose 昭和大学, 歯学部, 助教 (30806576)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 脂肪組織由来再生細胞 / 軟骨組織 / ADRCs

Outline of Final Research Achievements

Adipose tissue-derived regenerated cells (ADRCs) were investigated for the aim of improving bone and cartilage defects. ADRCs had pluripotent capacity of differentiating into osteoblast-like cells and chondrocyte-like cells. Assuming a model of articular disc or temporomandibular joint injury, an atelocollagen sponge infused with ADRCs was transplanted into a rat knee joint in which the medial meniscus was partially resected. Compared with the sponge alone, the atelocollagen sponge with ADRCs improved the repair of cartilage-like tissue. In dynamic mechanical analysis, higher elastic moduli and lower fluid permeability of regenerated meniscal tissue demonstrated a favorable structure-function relationship required for native menisci. ADRCs will be useful in future clinical cell-based therapy strategies, including as a cell source for reconstruction of hard tissue.

Free Research Field

口腔外科学

Academic Significance and Societal Importance of the Research Achievements

脂肪組織から単離したADRCsは、骨芽細胞様細胞や軟骨細胞様細胞へと分化できる多分化能を有し、軟骨修復に大きく寄与した。骨髄などに比べ低侵襲かつ移植時に新鮮な細胞が十分量採取できるADRCsは、硬組織再生医療の細胞ソースとして有用と考える。