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2021 Fiscal Year Annual Research Report

人工核酸LNAを用いた日本人集団における高感度メチル化解析と年齢推定への応用

Research Project

Project/Area Number 19K19477
Research InstitutionAsahikawa Medical College

Principal Investigator

磯崎 翔太郎  旭川医科大学, 大学病院, 医員 (60834504)

Project Period (FY) 2019-04-01 – 2022-03-31
Keywordsメチル化
Outline of Annual Research Achievements

日本人集団70名から抽出したDNAに対し、7領域(PTPN7, SST, CNGA3, KLF14, TSSK6, TBR1, and SLC12A5)のCpG配列のメチル化率を一塩基伸長法により測定し、重回帰分析を用いることで平均絶対偏差4.88歳の法医学的年齢推定法を確立した。本法におけるマルチプレックスPCRの段階において、各Primerの5'末端側にLNAを2塩基導入したPrimerを設計し、0%,50%,100%のメチル化率のstandard DNA 10ng に対してメチル化率測定を行い、LNA未導入のPrimerを用いた場合と比較した。その結果、LNA導入のprimerを使用した場合、7領域中6領域でキャピラリー電気泳動時の相対蛍光単位の増高が認められた。2021年度に本研究成果を日本DNA多型学会第30回学術集会で発表した。

  • Research Products

    (6 results)

All 2022 2021

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 1 results) Presentation (2 results)

  • [Journal Article] Soluble thrombomodulin ameliorates aberrant hemostasis after rewarming in a rat accidental hypothermia model2022

    • Author(s)
      Takauji Shuhei、Tanaka Hiroki、Hayakawa Mineji、Horioka Kie、Isozaki Shotaro、Konishi Hiroaki
    • Journal Title

      Biochemical and Biophysical Research Communications

      Volume: 587 Pages: 1~8

    • DOI

      10.1016/j.bbrc.2021.11.086

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Hypoxia-induced nuclear translocation of β-catenin in the healing process of frostbite2022

    • Author(s)
      Isozaki Shotaro、Tanaka Hiroki、Horioka Kie、Konishi Hiroaki、Kashima Shin、Takauji Shuhei、Fujiya Mikihiro、Druid Henrik
    • Journal Title

      Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease

      Volume: 1868 Pages: 166385~166385

    • DOI

      10.1016/j.bbadis.2022.166385

    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Probiotic Aspergillus oryzae produces anti-tumor mediator and exerts anti-tumor effects in pancreatic cancer through the p38 MAPK signaling pathway2021

    • Author(s)
      Konishi Hiroaki、Isozaki Shotaro、Kashima Shin、Moriichi Kentaro、Ichikawa Satoshi、Yamamoto Kazuki、Yamamura Chikage、Ando Katsuyoshi、Ueno Nobuhiro、Akutsu Hiroaki、Ogawa Naoki、Fujiya Mikihiro
    • Journal Title

      Scientific Reports

      Volume: 11 Pages: 11070

    • DOI

      10.1038/s41598-021-90707-4

    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Treatment of hepatocellular carcinoma with autologous platelets encapsulating sorafenib or lenvatinib: A novel therapy exploiting tumor‐platelet interactions2021

    • Author(s)
      Tanaka Hiroki、Horioka Kie、Hasebe Takumu、Sawada Koji、Nakajima Shunsuke、Konishi Hiroaki、Isozaki Shotaro、Goto Masanori、Fujii Yumiko、Kamikokura Yuki、Ogawa Katsuhiro、Nishikawa Yuji
    • Journal Title

      International Journal of Cancer

      Volume: 150 Pages: 1640~1653

    • DOI

      10.1002/ijc.33915

    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 人工核酸導入Primerを用いた高感度メチル化解析と法医学的年齢推定2021

    • Author(s)
      磯崎翔太郎
    • Organizer
      日本DNA多型学会第30回学術集会
  • [Presentation] The assessment of cutaneous epithelium hypoxia in the murine frostbite model2021

    • Author(s)
      Shotaro Isozaki, Hiroki Tanaka, Kie Horioka, Hiroaki Konishi, Shin Kashima, Henrik Druid
    • Organizer
      第105次日本法医学会学術全国集会

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Published: 2022-12-28  

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