DNA methylation-based forensic age prediction in Japanese populations using locked nucleic acid.

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K19477
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 58040:Forensics medicine-related
• Research Institution: Asahikawa Medical College
• Principal Investigator: Isozaki Shotaro 旭川医科大学, 大学病院, 医員 (60834504)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: Locked Nucleic Acid

Outline of Final Research Achievements

In this study, we developed the forensic age estimation method of the Japanese population by measuring the methylation rate of DNA using a single-base extension reaction. In addition, we succeeded in increasing the sensitivity by introducing Locked Nucleic Acid （LNA) into each primers.

Free Research Field

法医学

Academic Significance and Societal Importance of the Research Achievements

本研究成果は、微量な試料からのDNAメチル化率に基づく年齢推定法として法医実務へ利用可能である。また、次世代シーケンサーやパイロシーケンサーを必要とせず、コストパフォーマンスに優れた方法でありその有用性は高い。