2020 Fiscal Year Final Research Report
Subclassification of Y chromosome haplogroups in Japanese population and application to personal identification
Project/Area Number |
19K19489
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Research Category |
Grant-in-Aid for Early-Career Scientists
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Allocation Type | Multi-year Fund |
Review Section |
Basic Section 58040:Forensics medicine-related
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Research Institution | Tokai University |
Principal Investigator |
OCHIAI Eriko 東海大学, 医学部, 助教 (60760270)
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Project Period (FY) |
2019-04-01 – 2021-03-31
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Keywords | Y染色体ハプログループ / Y-SNP / Y-STR / 個人識別 |
Outline of Final Research Achievements |
In order to use Y-chromosome haplogroups for forensic applications such as personal identification, high discrimination power is required, and further subclassification of the lineage is required. We examined the frequency of Y haplogroups in the Japanese population and attempted to subdivide them including downstream subgroups. The results of the classification of 567 Japanese males showed that they were largely divided into C, D1, N, O*, O1b, O2, and Q lineages. The O1b lineage was further subdivided into six types: O1b*, O1b2a*, O1b2a1a1*, O1b2a1a1a, O1b2a1a1b, and O1b2a1a1c. Sequence analysis of the 32 newly selected Y-SNPs loci using next-generation sequencers showed that the discriminatory ability of the D lineage samples was greatly improved.
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Free Research Field |
法医学
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Academic Significance and Societal Importance of the Research Achievements |
法医実務でのDNA鑑定では微量試料や陳旧化のため断片化したDNAを扱うことがあり、通常の検査で用いるSTR(反復配列の多型)の鑑定結果が十分に得られない場合がある。増幅産物サイズの小さいSNP解析であれば検出できる可能性があるため、Y-SNPの識別能力が向上することで個人識別への利用が期待される。理論上では数十座位のY-SNPの分析で下流サブグループまで判定することができるため、例えば性犯罪事例における微量試料の鑑定を行うときに被疑者の絞り込みや冤罪の防止にも繋がると考えられる。民族推定の目的でもYハプログループの詳細な分類は有用であり、試料の所属集団を調べる際にも役に立つ。
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