2023 Fiscal Year Final Research Report
Development of the model to predict PCR byproduct generation in STR typing.
| Project/Area Number |
19K19492
|
|---|
| Research Category |
Grant-in-Aid for Early-Career Scientists
|
| Allocation Type | Multi-year Fund |
|---|
| Review Section |
Basic Section 58040:Forensics medicine-related
|
|---|
| Research Institution | National Research Institute of Police Science |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2019-04-01 – 2024-03-31
|
| Keywords | スタター / フラグメント解析 / 合成DNA |
|---|
| Outline of Final Research Achievements |
In this study, three main results were obtained: 1. Establishment of a system to measure the stutter ratio and stutter generation ratio of synthetic oligonucleotides, 2. Differences in stutter ratios depending on the order of bases in repeat sequences, and 3. The possibility that stutter generation ratio is not constant across PCR cycles. Of these, the results of 2 and 3 were not initially expected. In particular, the result of 2 strongly suggests that the conventional theory, "the higher AT content of repeat sequence, the higher the stutter ratio", dose not apply to 4 base repeat sequences, but is affected by the order of the bases. This is a very impactful new finding.
|
| Free Research Field |
法医学
|
| Academic Significance and Societal Importance of the Research Achievements |
現在、STR型検査におけるスタターの性質の詳細及び発生メカニズムについては、理論面において理解が進んでいないため、実務においては多数の試料の実測値からその性質を推定してスタターを扱っている。本研究の成果は、スタターの性質の詳細及び発生メカニズムに関する新しい知見であり、かつ従来の知見が当てはまらないことを示したものである。スタターの詳細について理解が進むことはSTR型検査、ひいてはDNA型鑑定の理論面のさらなる解明につながることから、本研究の成果は学術的意義・社会的意義の大きいものであると考えている。
|
