Dynamics of interstitial oxygen partial pressure in skeletal muscle of ECMO model rats

2020 Fiscal Year Final Research Report

• Project/Area Number: 19K19806
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59010:Rehabilitation science-related
• Research Institution: Niigata University of Health and Welfare
• Principal Investigator: Hotta Kazuki 新潟医療福祉大学, リハビリテーション学部, 講師 (30791248)
• Project Period (FY): 2019-04-01 – 2021-03-31
• Keywords: 骨格筋 / 酸素 / ECMO

Outline of Final Research Achievements

Extracorporeal membrane oxygenation （ECMO） is currently used as a life-saving treatment for patients with severe cardiopulmonary disease. This study aims to reveal the effect of ECMO on pO2 in artery and skeletal muscle. Tibialis anterior muscle was exposed under isoflurane aneshesia. Phosphorescence quenching techniques were used to measure pO2 in muscle interstitium of sham and ECMO model rats. Arterial partial pressure of oxygen (PaO2) was significantly increased in ECMO model rats as compared to the sham. However, interstitial pO2 did not change during ECMO as compared to the sham. Therefore, ECMO did not likely affect the interstitial pO2 in rat skeletal muscle.

Free Research Field

微小循環

Academic Significance and Societal Importance of the Research Achievements

ECMOは重症な呼吸循環不全患者を救命する最後の砦である．救命された後の臨床的問題として，日常生活への復帰が骨格筋萎縮によって阻害されることである．骨格筋の低酸素あるいは高酸素は，日常生活の自立を阻害する因子であり，ECMOは動脈血を高酸素化する．本研究により，ECMOは骨格筋の近藤先生には影響しないことが明らかとなった．したがって，ECMO管理によって生ずる筋萎縮は酸素動態以外の炎症や酸化ストレスなどが影響している可能性がある．