The mechanism by which immobilization decreases GLUT1-dependent glucose uptake in rat skeletal muscle

2021 Fiscal Year Final Research Report

• Project/Area Number: 19K20031
• Research Category: Grant-in-Aid for Early-Career Scientists
• Allocation Type: Multi-year Fund
• Review Section: Basic Section 59020:Sports sciences-related
• Research Institution: Nagaoka National College of Technology
• Principal Investigator: Kawamoto Emi 長岡工業高等専門学校, 物質工学科, 准教授 (40634514)
• Project Period (FY): 2019-04-01 – 2022-03-31
• Keywords: immobilization / soleus muscle / GLUT1 / TXNIP

Outline of Final Research Achievements

Acute short duration of disuse decreases insulin-independent glucose uptake in skeletal muscle, but the mechanisms is not identified. In this study, we focused on GLUT1, that is a glucose transport protein responsible for basal glucose uptake, and Thioredoxin interacting protein (TXNIP) that is in relative to glucose metabolism. As a result, TXNIP was expressed and increased in plasma membrane of immobilized soleus muscle. However, GLUT1 and GLUT4 protein expression was not different between both Non-immobilized and Immobilized rat soleus muscle. Finally, this study suggested that TXNIP is a key factor for regulating GLUT.

Free Research Field

運動生理学

Academic Significance and Societal Importance of the Research Achievements

日常生活では就寝やオフィスワークのように、GLUT1依存的な血糖取り込みが主体の時間が1日を通じて多くある。したがって、本研究において、身体活動量が減少した筋（不活動筋）のGLUT1依存的な血糖取り込みが低下するメカニズムを示唆することで、寝たきりやフレイル、不活発な日常生活等、不活動由来の代謝疾患を予防・改善する薬剤、運動や栄養処方の開発に貢献する。また身体活動の重要性を示す科学的根拠となる。